Bioprospecting of Marine Fungi from Coastal Karnataka Region as Potential Source of Economically Important Enzyme L-Glutaminase and their Comparative Genomic Study

Marine fungi are important sources of new metabolites including certain enzymes medical interest due to their enormous capacity adapt themselves extreme environments. Living in a highly competitive ecological niche, they produce unusual chemical moieties. biological resources green, abundant, renewable and aid economic development. The present study investigates the production L-Glutaminase which is therapeutic industrial importance, from marine coastal Karnataka. Primary screening on agar plates submerged fermentation broth was employed for enzyme production. Both yeasts (Pichia sp) filamentous fungal strains (Aspergillus, Penicillium) were found be efficient producers L-Glutaminase. Of 42 isolates, five potential selected through primary Thin Layer Chromatography performed confirm Filamentous identified morphological molecular methods as Penicillium Aspergillus with 99-100% similarity. A. foveolatus (MT667385)and nidulans (MT667422) (1.58U/ml 1.41IU/ml). yeast Pichia kudriavzevii (MT667428), moderate producer Glutaminase first reported this Neosartorya quadricincta (MT667427) P. citrinum (MT667426) also producers. After fungi, isolated strains’ confirmed using SDS PAGE, FTIR Mass analysis. This emphasizes necessity culturing scope use these further commercial would uplift economy.